1UB4
crystal structure of MazEF complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97899 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.591, 116.717, 76.841 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.000 * - 1.700 |
| R-factor | 0.248 |
| Rwork | 0.210 |
| R-free | 0.23200 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.720 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.037 * | 0.187 |
| Total number of observations | 805050 * | |
| Number of reflections | 37150 | |
| <I/σ(I)> | 26.7 | 13.6 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 21.7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 4 * | NaAcetate, NaCl, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.5 |
| 3 | 1 | reservoir | 2 (M) | ||
| 4 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) |
