1UAS
Crystal structure of rice alpha-galactosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2002-01-31 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.656, 71.420, 86.238 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.500 |
| R-factor | 0.16 |
| Rwork | 0.160 |
| R-free | 0.17800 |
| Structure solution method | MIR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.310 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.055 | 0.330 |
| Number of reflections | 61397 * | 6311 * |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 2-propanol, ammonium sulfate, acetate, D-galactose, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | 2-propanol | 5 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
| 4 | 1 | reservoir | acetate | 0.1 (M) | pH4.5 |
| 5 | 1 | reservoir | D-galactose | 5 (%) |
