1UAM

Crystal structure of tRNA(m1G37)methyltransferase: Insight into tRNA recognition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-10-10
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	92.900, 92.900, 179.971
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.200
R-factor	0.22
Rwork	0.220
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	22.500 *
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.069 *	0.262 *
Total number of observations	39786 *
Number of reflections	15004
<I/σ(I)>	7.4	2.3
Completeness [%]	98.5	91.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6	295	sodium citrate, ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K