1UAK

Crystal structure of tRNA(m1G37)methyltransferase: Insight into tRNA recognition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9500
Spacegroup name	H 3 2
Unit cell lengths	93.562, 93.562, 177.971
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.050
R-factor	0.176
Rwork	0.176
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	22.200 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.160
High resolution limit [Å]	2.050	2.050
Rmerge	0.105 *
Total number of observations	114230 *
Number of reflections	18936
<I/σ(I)>	6	2.3
Completeness [%]	99.3	99.3
Redundancy		0.321 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	295	sodium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K