1UAJ
Crystal structure of tRNA(m1G37)methyltransferase: Insight into tRNA recognition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9500 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 98.047, 98.047, 176.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 1.850 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 * | 0.353 * |
| Total number of observations | 216789 * | |
| Number of reflections | 28115 | |
| <I/σ(I)> | 9.3 | 2.2 |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 295 | sodium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
