1UAI
Crystal Structure of the Alginate Lyase from Corynebacterium sp.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.925, 47.234, 50.307 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 34.440 - 1.200 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.19000 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.056 | 0.119 |
| Number of reflections | 59499 | |
| <I/σ(I)> | 16.1 | 13.2 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
