1UAG
UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 100 |
| Detector technology | CDD |
| Collection date | 1996-06 |
| Spacegroup name | P 41 |
| Unit cell lengths | 65.428, 65.428, 134.438 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.950 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.23500 |
| Structure solution method | MAD AND MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.500 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MADSYS |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.970 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.860 |
| Rmerge | 0.065 * | 0.259 * |
| Number of reflections | 41111 | |
| <I/σ(I)> | 8.7 | 2.03 |
| Completeness [%] | 94.7 * | 79.7 * |
| Redundancy | 3.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 15 * | Auger, G., (1998) Protein Expr. Purif., 13, 23. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.8 (mg/ml) | |
| 2 | 1 | drop | UMA | 1 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | sodium HEPES | 20 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.6-1.8 (M) | |
| 7 | 1 | reservoir | sodium HEPES | 100 (mM) |
