1UAD
Crystal structure of the RalA-GppNHp-Sec5 Ral-binding domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | I 4 |
Unit cell lengths | 117.418, 117.418, 102.676 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.510 - 2.100 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ctq |
RMSD bond length | 0.006 |
RMSD bond angle | 1.143 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.044 * | 0.274 * |
Total number of observations | 249726 * | |
Number of reflections | 40357 | |
<I/σ(I)> | 40.4 | 3.75 |
Completeness [%] | 99.4 | 98.6 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 20 * | PEG8000, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 13-17 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.5 |
3 | 1 | reservoir | PEG8000 | 7.5-8.5 (%) |