1UAC
Crystal Structure of HYHEL-10 FV MUTANT SFSF Complexed with TURKEY WHITE LYSOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-07 |
| Detector | FUJI |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 |
| Unit cell lengths | 67.150, 67.150, 77.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.149 |
| Rwork | 0.149 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.056 | 0.375 |
| Number of reflections | 36020 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 96.7 | 95.2 |
| Redundancy | 3.8 * | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | Kondo, H., (1999) J. Biol. Chem., 274, 27623. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 4 | 1 | reservoir | tri-sodium citrate dihydrate | 100 (mM) | pH6.6 |
| 5 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 6 | 1 | reservoir | ethylene gylcol | 10 (%) |
