1UAA
E. COLI REP HELICASE/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 141.800, 141.800, 284.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.228 * |
| Rwork | 0.228 |
| R-free | 0.32800 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.918 * |
| Data reduction software | DENZO |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.053 |
| Total number of observations | 434546 * |
| Number of reflections | 57472 |
| Completeness [%] | 97.3 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 20 * | pH 8.00, VAPOR DIFFUSION, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | TRIS-HCL | ||
| 4 | 1 | 1 | EDTA | ||
| 5 | 1 | 1 | GLYCEROL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | protain | 20 (mg/ml) | |
| 6 | 1 | drop | PEG8000 | 2.5 (%) | |
| 7 | 1 | reservoir | glycerol | 2 (%(v/v)) |
