1U9Z
Crystal Structure of Phosphoribosyl Diphosphate Synthase Complexed with AMP and Ribose 5-Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.087 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.260, 138.250, 138.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.800 |
| R-factor | 0.24 |
| Rwork | 0.238 |
| R-free | 0.27200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | Apo structure of Methanocaldococcus jannaschii PRPP synthase PDBID 1u9y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.296 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.105 | 0.573 |
| Number of reflections | 34117 | |
| <I/σ(I)> | 10.5 | 1.9 |
| Completeness [%] | 99.5 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
