1U9U
Crystal structure of F58Y mutant of cytochrome b5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | CONVENTIONAL Cu |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2001-11-30 |
| Detector | BRUKER |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.515, 40.405, 39.300 |
| Unit cell angles | 90.00, 111.70, 90.00 |
Refinement procedure
| Resolution | 36.520 - 1.860 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.21500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ehb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.100 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 7423 | |
| Completeness [%] | 82.3 | 46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | phosphate buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
