1U9T

Crystal Structure Analysis of ChuS, an E. coli Heme Oxygenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-30
Detector	ADSC QUANTUM 210
Wavelength(s)	0.979499
Spacegroup name	P 1 21 1
Unit cell lengths	41.684, 57.880, 59.578
Unit cell angles	90.00, 96.02, 90.00

Refinement procedure

Resolution	41.460 - 2.160
R-factor	0.207
Rwork	0.207
R-free	0.28300
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.400
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	14606
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	PEG 3500, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	6	298	PEG 3500, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	6	298	PEG 3500, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K