1U9P
Permuted single-chain Arc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Detector technology | CCD |
| Collection date | 2003-08-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.181, 62.916, 30.380 |
| Unit cell angles | 90.00, 102.69, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.253 |
| Rwork | 0.249 |
| R-free | 0.29700 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 8500 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 95.0 | 75.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 3350, sodium fluoride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 3350, sodium fluoride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
