1U9M
Crystal structure of F58W mutant of cytochrome b5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2002-12-02 |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.044, 87.484, 138.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.550 - 2.000 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ehb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.095 | 0.571 |
| Number of reflections | 40502 | |
| Completeness [%] | 87.3 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | phosphate buffer, dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
