1U9L

Structural basis for a NusA- protein N interaction

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-11
Detector	MARRESEARCH
Wavelength(s)	0.95, 1.05
Spacegroup name	P 43 21 2
Unit cell lengths	69.787, 69.787, 67.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.840 - 1.900
R-factor	0.21659
Rwork	0.216
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.231
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.850	1.850
Number of reflections	13388
<I/σ(I)>	17.8	1.3
Completeness [%]	98.5	99.4
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	Ammonium sulfate, MES, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K