1U9F

Heterocyclic Peptide Backbone Modification in GCN4-pLI Based Coiled Coils: Replacement of K(15)L(16)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-04-30
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	67.599, 67.599, 86.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.490 - 2.200
R-factor	0.25237
Rwork	0.249
R-free	0.31977
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.928
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.490	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.050	0.314
Number of reflections	10727
<I/σ(I)>	7.4	2.3
Completeness [%]		99.5
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.085M HEPES Na, pH7.5, 8.5% v/v isopropanol, 17% w/v PEG4000, 15% v/v anhydrous glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K