1U9C
Crystallographic structure of APC35852
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-04-12 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97970 |
| Spacegroup name | P 43 |
| Unit cell lengths | 66.183, 66.183, 53.905 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.350 |
| R-factor | 0.1734 |
| Rwork | 0.172 |
| R-free | 0.19729 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1RW7 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.849 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.236 | 0.368 |
| Number of reflections | 49550 | |
| <I/σ(I)> | 53.7 | 4.1 |
| Completeness [%] | 96.2 | 72.8 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 0.2M magnesium sulfate heptahydrate, 20% w/v PEG 3350, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
