1U9B
MURINE/HUMAN UBIQUITIN-CONJUGATING ENZYME UBC9
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 281 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-26 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 35.390, 93.950, 115.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.185 * |
| Rwork | 0.185 |
| R-free | 0.25200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1AAK |
| RMSD bond length | 0.015 |
| RMSD bond angle | 16.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | TNT (5D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.020 |
| Rmerge | 0.058 | 0.410 |
| Number of reflections | 12854 | |
| <I/σ(I)> | 17 | 3 |
| Completeness [%] | 98.0 | 99 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 23% PEG MONOMETHYL ETHER 5000,9% ISOPROPANOL, 0.1 M AMMONIUM SULFATE, 0.1 M MES BUFFER, PH6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.5 (mg/ml) | |
| 10 | 1 | reservoir | isopropanol | 9 (%) | |
| 11 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
| 12 | 1 | reservoir | MES | 0.1 (M) | |
| 2 | 1 | drop | 75 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 0.25 (mM) | |
| 4 | 1 | drop | HEPES | 5 (mM) | |
| 5 | 1 | drop | PEG5000 | 11.5 (%) | |
| 6 | 1 | drop | isopropanol | 4.5 (%) | |
| 7 | 1 | drop | ammonium sulfate | 0.05 (M) | |
| 8 | 1 | drop | MES | 0.05 (M) | |
| 9 | 1 | reservoir | PEG5000 | 23 (%) |
