1U90

Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-13
Temperature [K]	277
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	52.590, 61.535, 112.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.670 - 2.000
Rwork	0.189
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u8y
RMSD bond length	0.005
RMSD bond angle	1.100
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.700
High resolution limit [Å]	1.900
Number of reflections	25805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K