1U8Z
Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.611, 62.059, 112.664 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.760 - 1.500 |
Rwork | 0.202 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u90 |
RMSD bond length | 0.005 |
RMSD bond angle | 2.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.800 |
High resolution limit [Å] | 1.500 |
Number of reflections | 60664 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |