1U7Z
Phosphopantothenoylcysteine synthetase from E. coli, 4'-phosphopantothenoyl-CMP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 43.627, 142.584, 244.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.203 |
| Rwork | 0.196 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u7u |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.670 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.088 | 0.297 |
| Number of reflections | 32347 | |
| Completeness [%] | 93.4 | 97.1 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | PEG 3000, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
