1U7X
crystal structure of a mutant M. jannashii tyrosyl-tRNA synthetase specific for O-methyl-tyrosine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96860 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.371, 185.000, 93.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.250 - 3.000 |
| R-factor | 0.237 |
| Rwork | 0.229 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u7d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.250 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 14836 | |
| <I/σ(I)> | 7 | 1.7 |
| Completeness [%] | 91.0 | 89 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 5 | 277 | 12-15% PEG4000, 0.2M ammonium sulfate, 0.2M sodium acetate, , pH 5.0, LIQUID DIFFUSION, temperature 277K |
