1U7O

Magnesium Dependent Phosphatase 1 (MDP-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-08-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	59.405, 59.405, 99.557
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.420 - 1.900
R-factor	0.214
Rwork	0.200
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MAD phased structure at lower resolution
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.420	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	14675
<I/σ(I)>	20.8
Completeness [%]	99.7	99.7
Redundancy	5.5	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	PEG 4000, sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K