1U7G

Crystal Structure of Ammonia Channel AmtB from E. Coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-14
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1159
Spacegroup name	P 63
Unit cell lengths	96.541, 96.541, 94.622
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.400
R-factor	0.137
Rwork	0.133
R-free	0.16800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u77
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.450
High resolution limit [Å]	1.350	1.400
Rmerge	0.058	0.621
Number of reflections	104957
<I/σ(I)>	19.3	1.6
Completeness [%]	95.7	90.4
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K