1U7C
Crystal Structure of AmtB from E.Coli with Methyl Ammonium.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1159 |
Spacegroup name | P 63 |
Unit cell lengths | 95.700, 95.700, 94.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.850 |
R-factor | 0.196 |
Rwork | 0.195 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u77 |
RMSD bond length | 1.300 |
RMSD bond angle | 0.007 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.053 | 0.688 |
Number of reflections | 41018 | |
<I/σ(I)> | 18.6 | 2.1 |
Completeness [%] | 96.7 | 90 |
Redundancy | 14.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |