1U77

Crystal Structure of Ammonia Channel AmtB from E. Coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-24
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1159, 1.0080, 0.9797
Spacegroup name	P 63
Unit cell lengths	95.768, 95.768, 94.664
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.1843
Rwork	0.182
R-free	0.20500
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.064	0.695
Number of reflections	29244
<I/σ(I)>	25.8	2
Completeness [%]	87.4	48.2
Redundancy	19.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K