1U6M

The crystal structure of acetyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-24
Detector	MARRESEARCH
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	115.257, 115.257, 225.257
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	102.600 - 2.400
R-factor	0.20558
Rwork	0.203
R-free	0.24676
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	2.143
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400	2.400
Rmerge	0.119
Number of reflections	59682
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	2.4M Ammonium Sulfate, 05M sodium Citrate, cryo Glycerol or Sucrose, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K