1U6L

Crystal structure of protein PA1353 from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	70
Detector technology	CCD
Collection date	2004-06-24
Detector	MARRESEARCH
Wavelength(s)	0.97900
Spacegroup name	P 21 21 21
Unit cell lengths	56.579, 76.294, 82.193
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.810
R-factor	0.1999
Rwork	0.197
R-free	0.25712
Structure solution method	SAD
RMSD bond length	0.031
RMSD bond angle	2.533
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.111
Number of reflections	9121
Completeness [%]		99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	30% PEG 6000, 1M Lithium Chloride, 0.1M sodium Acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K