1U6D

Crystal structure of the Kelch domain of human Keap1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-06-12
Detector	RIGAKU RAXIS IV
Spacegroup name	P 65 2 2
Unit cell lengths	85.892, 85.892, 148.753
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.880 - 1.850
R-factor	0.14941
Rwork	0.148
R-free	0.18237
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	1.257
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.081	0.520
Number of reflections	28169
<I/σ(I)>	33.9	4.2
Completeness [%]	99.9	100
Redundancy	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	4% PEG 4000, 100 mM Na Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K