1U69
Crystal Structure of PA2721 Protein of Unknown Function from Pseudomonas aeruginosa PAO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2004-05-31 |
| Detector | SBC-2 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.546, 55.017, 67.069 |
| Unit cell angles | 70.71, 89.88, 85.61 |
Refinement procedure
| Resolution | 63.250 - 1.600 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.168 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.800 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 79080 | |
| Completeness [%] | 89.0 | 46.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | 0.1M Sodium Cacodylate, 0.2M Ammonium Sulfate, 30% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.50 |
