1U5Y
Crystal structure of murine APRIL, pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.344, 81.166, 53.585 |
| Unit cell angles | 90.00, 111.79, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.20802 |
| Rwork | 0.201 |
| R-free | 0.26805 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | I23 structure of APRIL |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.358 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 23803 | |
| <I/σ(I)> | 14.7 | 4.3 |
| Completeness [%] | 94.5 | 93 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | 0.1 M Tris pH 8.0, 1M LiCl, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
