1U5U
The structure of an Allene Oxide Synthase reveals a novel use for a catalase fold
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-17 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.940, 76.679, 77.761 |
| Unit cell angles | 90.00, 109.66, 90.00 |
Refinement procedure
| Resolution | 34.830 - 2.000 |
| R-factor | 0.209 |
| Rwork | 0.196 |
| R-free | 0.23900 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.830 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 104610 | |
| <I/σ(I)> | 21.5 | 3.7 |
| Completeness [%] | 95.5 | 88 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | 298 | PEG MME 5000, bis-tris, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 5.70 |
