1U5T

Structure of the ESCRT-II endosomal trafficking complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Collection date	2004-02-12
Spacegroup name	P 43 2 2
Unit cell lengths	150.040, 150.040, 185.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.600
R-factor	0.2362
Rwork	0.226
R-free	0.28850
Structure solution method	MIR
RMSD bond length	0.165
RMSD bond angle	2.557
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000
High resolution limit [Å]	3.600
Number of reflections	41210
Completeness [%]	91.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	Naacetate, hepes, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K