1U5Q
Crystal Structure of the TAO2 Kinase Domain: Activation and Specifity of a Ste20p MAP3K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-19 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.96417 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 186.220, 186.220, 94.506 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
Rwork | 0.223 |
R-free | 0.26890 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.707 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.311 | |
Number of reflections | 54996 | |
<I/σ(I)> | 30 | 4 |
Completeness [%] | 97.3 | 98.2 |
Redundancy | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Imidazole, PEG 1000, calcium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |