1U5Q

Crystal Structure of the TAO2 Kinase Domain: Activation and Specifity of a Ste20p MAP3K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-19
Detector	CUSTOM-MADE
Wavelength(s)	0.96417
Spacegroup name	P 65 2 2
Unit cell lengths	186.220, 186.220, 94.506
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.100
Rwork	0.223
R-free	0.26890
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.707
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge		0.311
Number of reflections	54996
<I/σ(I)>	30	4
Completeness [%]	97.3	98.2
Redundancy	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Imidazole, PEG 1000, calcium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K