1U5P
Crystal Structure of Repeats 15 and 16 of Chicken Brain Alpha Spectrin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794, 1.0721, 1.0494 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 148.200, 148.200, 148.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.18934 |
| Rwork | 0.187 |
| R-free | 0.22479 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.911 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.513 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 | 0.314 |
| Number of reflections | 38078 | |
| <I/σ(I)> | 7.6 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 287 | 1M NaH2PO4, 1M K2HPO4, 0.2M ammonium acetate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
