1U5K
Recombinational repair protein RecO
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Detector technology | CCD |
| Collection date | 2004-03-05 |
| Detector | SBC-1 |
| Wavelength(s) | 1.28309 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.967, 52.240, 100.683 |
| Unit cell angles | 90.00, 107.09, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.19879 |
| Rwork | 0.198 |
| R-free | 0.21345 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.744 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.046 | 0.553 |
| Number of reflections | 45542 | |
| <I/σ(I)> | 20 | 2.2 |
| Completeness [%] | 97.8 | 94.6 |
| Redundancy | 2.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | PEG4000, MgCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | PEG4000, MgCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
