1U5J
Propionibacterium shermanii transcarboxylase 5S subunit, Met186Ile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2004-06-02 |
| Detector | BRUKER PROTEUM |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.680, 147.042, 78.812 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.240 - 2.800 |
| R-factor | 0.214 |
| Rwork | 0.207 |
| R-free | 0.26800 |
| Structure solution method | Isomorphous replacement |
| Starting model (for MR) | 1rqb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | PROTEUM PLUS ((BRUKER)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | 0.224 |
| Number of reflections | 14015 | |
| <I/σ(I)> | 5.6 | 2.7 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 4000, Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
