1U5I
Crystal Structure analysis of rat m-calpain mutant Lys10 Thr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5412 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.658, 156.520, 64.227 |
| Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.860 |
| R-factor | 0.19605 |
| Rwork | 0.191 |
| R-free | 0.28333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.064 |
| RMSD bond angle | 3.891 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.700 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Number of reflections | 25795 | |
| Completeness [%] | 95.0 | 90 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | PEG 6000, MES, sodium chloride, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
