1U5G
Crystal Structure of the PH Domain of SKAP-Hom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.613, 65.695, 143.767 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.100 |
| R-factor | 0.216 |
| Rwork | 0.213 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Crystal Structure of the PH Domain of SKAP-Hom with Vector-derived N-terminal Linker Peptide PDB entry 1U5F. |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.337 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.052 | 0.379 |
| Number of reflections | 31477 | |
| <I/σ(I)> | 16.3 | 2.9 |
| Completeness [%] | 93.1 | 97.9 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | PEG 4000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
