1U5E

Crystal Structure of a N-terminal Fragment of SKAP-Hom Containing Both the Helical Dimerization Domain and the PH Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-06
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	106.205, 56.742, 89.115
Unit cell angles	90.00, 95.82, 90.00

Refinement procedure

Resolution	35.000 - 2.600
R-factor	0.181
Rwork	0.179
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	The PH Domain of SKAP-Hom PDB entry 1u5g
RMSD bond length	0.050
RMSD bond angle	3.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.660
High resolution limit [Å]	2.600	2.600
Rmerge	0.059	0.390
Number of reflections	15738
<I/σ(I)>	11.9	2
Completeness [%]	95.5	99.3
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	298	PEG 4000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K