1U58
Crystal structure of the murine cytomegalovirus MHC-I homolog m144
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.400, 51.200, 71.700 |
| Unit cell angles | 90.00, 105.70, 90.00 |
Refinement procedure
| Resolution | 41.140 - 1.900 |
| Rwork | 0.211 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a6z |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.110 | 0.170 |
| Number of reflections | 36627 | |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 98.0 | 95.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 20K, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
