1U4Q

Crystal Structure of Repeats 15, 16 and 17 of Chicken Brain Alpha Spectrin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-02
Detector	MARRESEARCH
Wavelength(s)	1.005, 0.9785, 0.9633
Spacegroup name	C 1 2 1
Unit cell lengths	150.030, 74.860, 66.610
Unit cell angles	90.00, 97.70, 90.00

Refinement procedure

Resolution	14.940 - 2.500
Rwork	0.223
R-free	0.31300
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.700	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.069	0.308
Number of reflections	36806
<I/σ(I)>	7.8	2.9
Completeness [%]	98.9	99
Redundancy	3.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	283	24% PEG 3.3K, 0.2M Lithium Sulfate, 2% MPD, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 283, 287K
1	VAPOR DIFFUSION, HANGING DROP	8.5	283	24% PEG 3.3K, 0.2M Lithium Sulfate, 2% MPD, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 283, 287K