1U40

IspF with 4-diphosphocytidyl-2C-methyl-D-erythritol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-04-08
Detector	MARRESEARCH
Wavelength(s)	1.541
Spacegroup name	I 21 3
Unit cell lengths	144.810, 144.810, 144.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.24
Rwork	0.233
R-free	0.26400
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1jy8
RMSD bond length	0.010
RMSD bond angle	1.850
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Rmerge	0.106
Number of reflections	12586
Completeness [%]	99.8
Redundancy	3.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	ammonium formate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K