1U3L
IspF with Mg and CDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.541 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 144.660, 144.660, 144.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.244 |
| Rwork | 0.240 |
| R-free | 0.26000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1jy8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.880 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.064 |
| Number of reflections | 17015 |
| Completeness [%] | 97.3 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | ammonium formiate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
