1U3F
Structural and Functional Characterization of a 5,10-Methenyltetrahydrofolate Synthetase from Mycoplasma pneumoniae (GI: 13508087)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.135, 74.369, 110.614 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.380 - 2.500 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 13398 | |
Completeness [%] | 99.2 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 30 mg/ml protein, 20% polyethylene glycol 4000, 5 mM adenosine triphosphate (ATP), pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |