1U2X
Crystal Structure of a Hypothetical ADP-dependent Phosphofructokinase from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | SBC-3 |
| Wavelength(s) | 0.96396, 0.97918, 0.97943 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.065, 99.932, 82.581 |
| Unit cell angles | 90.00, 110.38, 90.00 |
Refinement procedure
| Resolution | 39.840 - 2.000 |
| R-factor | 0.17678 |
| Rwork | 0.174 |
| R-free | 0.22592 |
| Structure solution method | MAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.985 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.445 |
| Number of reflections | 69682 | |
| <I/σ(I)> | 21 | 3.57 |
| Completeness [%] | 99.2 | 98.6 |
| Redundancy | 6.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 22% PEG4000, 0.1M Tris-HCl, 0.2M LiSO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
