1U2V
Crystal structure of Arp2/3 complex with bound ADP and calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.07018 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.300, 129.300, 204.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.550 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.280 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 90833 | |
| <I/σ(I)> | 24.1 | 6.8 |
| Completeness [%] | 99.4 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Hepes, potassium thiocyanate, PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
