1U2R
Crystal Structure of ADP-ribosylated Ribosomal Translocase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.089 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 119.725, 151.002, 65.172 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.600 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.26180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n0u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.334 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.056 | 0.257 |
| Number of reflections | 36997 | |
| <I/σ(I)> | 15.4 | 4.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 292 | PEG-8000, Ethylene Glycol, HEPES-NaOH, Magnesium Chloride, DTT, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
