1U2L
Crystal structure of the C-terminal domain from the catalase-peroxidase KatG of Escherichia coli (P1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.857, 52.764, 55.995 |
| Unit cell angles | 93.83, 90.71, 103.27 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| R-factor | 0.17863 |
| Rwork | 0.175 |
| R-free | 0.25097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u2k |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.942 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 25540 | |
| Completeness [%] | 97.9 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 34% PEG 4000, 50 mM Sodium Acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
